The PDQ System consists of the Liquid Chromatograph (high pressure pump, column oven and fixed wavelength detector), 10μL injection valve, printer, and pre-programmed GHb/A1c PDQ System software.
The PDQ systems and associated reagent kit is intended for in vitro determination of human glycated hemoglobin in samples of normal and diabetic patients.
Glycated haemoglobin (HbA1c) has a particular clinical interest in diabetes mellitus. Measurement of glycated haemoglobin is a clinically useful means of assessing glycemic control in diabetics.
Glycated haemoglobin values reflect blood glucose levels over the circulatory half-life of the erythrocyte and correlate significantly with mean blood glucose levels during that time.
Therefore, measurement of glycated haemoglobin provides a means, independent of multiple measurements such as patient records of self-monitored blood glucose, for assessing the overall efficacy of therapy.
Trinity Biotech HPLC instrumentation is available: small (PDQ SA: stand alone) and medium throughput (PDQ Plus, with an automated sample handling for 28 determinations).
Our products combine the speed and precision of HPLC with the accuracy of Boronate affinity columns, which eliminates false results due to interferences.
Boronate affinity chromatography is free from common interferences such as haemoglobin variants, non-glycation modifications and storage-related hemichromes. Boronate affinity chromatography requires no sample pretreatment to remove the labile component, since only stable (ketoamine-linked) GHb is retained by the boronate.
Compared to ion-exchange techniques, affinity separation is also less sensitive to quantitative errors caused by minor fluctuations in reagent pH and ionic strength.