|Order number||Description||Quantity||Delivery time||CE|
|TB 450-3||Bile Acid Stop Reagent||50 ml||1-3 days||x|
Bile Acid Stop Reagent
Trinity Biotech Bile Acids reagents are intended for the quantitative, enzymatic determination of bile acids concentration of serum at 530 nm.
Bile acids are present in most body fluids with the highest concentrations being found in bile. The major bile acids are cholic acid, chenodeoxycholic acid, deoxycholic acid and lithocholic acid. Bile acids, after conjugation with glycine or taurine and sometimes with glucuronide or sulfate esters, are secreted into bile and undergo an enterohepatic circulation. Serum bile acids originate from the intestinal absorption of bile acids. An increase in serum bile acids concentration in the fasting state or post-prandial is considered to be a specific indicator of liver disease. A decreased level indicates bile acid malabsorption, possibly due to ileal dysfunction. Bile acids concentration can be measured by chromatographic, spectrophotometric, or fluorometric procedures. They can also be determined by radioimmunoassay or similar competitive binding techniques, as well as by bioluminescence detection methods. Trinity Biotech Bile Acids reagents measure serum bile acids concentration enzymatically. The methodology is based essentially on the procedure of Mashige et al.